Working Group 2

Microalgae harvest and cell wall disruption

The objective of WG2 is to focus on the evaluation of innovative harvesting and cell disruption techniques in accordance to the targeted bioproducts.

Task A) Harvesting

Task B) Cell disruption


 

Harvesting

Cell disruption

 

Working Group Leader and Subleaders

Prof. Koenraad Muylaert

Prof. Koenraad Muylaert WG2 Leader

Prof. Koenraad Muylaert

WG2 Leader

Prof. Koenraad Muylaert

Country: Belgium

Institution: Aquatic Biology Lab, KU Leuven campus Kortrijk, KU Leuven University

Email: koenraad.muylaert@kuleuven.be

Website: www.kuleuven-kortrijk.be/biology

Key investigations:

  • Harvesting of microalgae based on flocculation (alkaline flocculation, bio-based flocculants)

  • Production of poly-unsaturated fatty acids and antioxidants from microalgae

  • Production of the natural pigment phycocyanin in Arthrospira

  • Control of grazers in microalgal cultures

  • Microalgae-based phosphorus removal and recovery from wastewaters

 

Key publications:

  • Vandamme, D., Beuckels, A., Vadelius, E., Depraetere, O., Noppe, W., Dutta, A., Foubert, I., Laurens, L. & Muylaert, K. (2016). Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris. Water research, 88, 301-307.

  • Eyley, S., Vandamme, D., Lama, S., Van den Mooter, G., Muylaert, K., & Thielemans, W. (2015). CO 2 controlled flocculation of microalgae using pH responsive cellulose nanocrystals. Nanoscale, 7(34), 14413-14421.

  • Depraetere, O., Pierre, G., Deschoenmaeker, F., Badri, H., Foubert, I., Leys, N., Wattiez, R. & Muylaert, K. (2015). Harvesting carbohydrate-rich Arthrospira platensis by spontaneous settling. Bioresource technology, 180, 16-21.

  • Beuckels, A., Smolders, E., & Muylaert, K. (2015). Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water research, 77, 98-106.

  • Vandamme, D., Foubert, I., & Muylaert, K. (2013). Flocculation as a low-cost method for harvesting microalgae for bulk biomass production. Trends in biotechnology, 31(4), 233-239.

 

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Dr Leen Bastiaens

Dr Leen Bastiaens Sub-leader WG2

Dr Leen Bastiaens

Sub-leader WG2

Dr Leen Bastiaens
Country: BelgiumInstitution: VITOEmail: leen.bastiaens@vito.beWebsite: www.vito.be Key investigations of VITO:

  • Membrane based algae harvesting & water recirculation
  • Cell disruption
  • Fractionation/extraction of crude extracts & purifications/conversion of crude fractions
  • Analytics
  • From lab to pilot scale

Key publications of VITO:

  • Gunerken E., E. D’hondt, M.H.M. Eppink, L. Garcia-Gonzalez, K. Elst, R.H. Wijffels. 2015. Cell disruption for microalgae biorefineries. Biotechnology advances. Volume 33, Issue 2, Pages 243-260
  • Servaes, K., M. Maesen, B. Prandi, S. Sforza, K. Elst.   Polar Lipid Profile of Nannochloropsis oculata Determined Using a Variety of Lipid Extraction Procedures. J. Agric. Food Chem. 63 (15):3931-3941.
  • De Baerdemaeker T., Lemmens B., Dotremont C., Fret J., Roef L., Goiris K., Diels L. 2013. Benchmark study on algae harvesting with backwashable submerged flat panel membranes. Biores. Technol., 129, 582-591.
  • Bijttebier, S.K.A., D’Hondt, E., Hermans, N., Apers, S. and S. Voorspoels. 2013. Unravelling ionization and fragmentation pathways of carotenoids using orbitrap technology: a first step towards identification of unknowns. Journal of Mass Spectrometry 48: 740-754.
  • Pilot infrastructure – http://www.sunbuilt.be/
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Working Group Members

Dr Sanda Velea

Dr Sanda Velea Leader Task a)

Dr Sanda Velea

Leader Task a)

Dr Sanda Velea

Country: Romania

Institution: National R&D Institute for Chemistry & Petrochemistry ICECHIM-Bucharest

Email: general.manager@icechim.ro

Website: http://www.icechim.ro

Key investigations:

  • Alternative bioresources
  • CO2 capture and sequestration by microalgae
  • photobioreactors
  • Algal biomass valorisation

 International patents:

  • Stepan Emil, Velea Sanda, Oancea Florin, Bomboş Mariana Mihaela, Vasilievici Gabriel, Pârvulescu Vasile, Blăjan Olimpiu, Crucean Augustin Constantin, “Process for obtaining aviation biofuel from microalgal biomass”, WO 2015/076687
  • Oancea Florin, Velea Sanda, Stepan Emil, Ilie Lucia, “Process for mixotrophic cultivation of algae”, WO 2015/076689
  • Oancea Florin, Velea Sanda, Ilie Lucia, Stepan Emil, “Nannochloris sp. stain for obtainment of bio-based aviation fuel”, WO 2015/072873

International conferences:

  • Emil Stepan, Sanda Velea, Olimpiu Blăjan, Elena-Emilia Oprescu, Gabriel Vasilievici, Elena Radu, Adrian Radu, “Bio-derived synthetic paraffinic kerosene from microalgae”, International Conference ”1st Chemistry in Energy Conference”, Edinburg, Marea Britanie,19-23 iulie 2015.
  • Oprescu E.E., Velea Sanda, Doncea S.M., Radu A., Emil Stepan, Bolocan I., „Biodiesel from algae oil with high free fatty acid over amphiphilic solid acid catalyst”, International Conference ICheaP12, 19-22.05.2015, Milano, Italia.
  • M. Popescu, I. Calinescu, Sanda Velea, D. Ighigeanu, D. Martin, Microwaves bio-effects on microalgae growth, International Conference Ampere 2015, Cracovia, Polonia, 14-17 september 2015.
  • Sanda Velea, S.B. Ghimis, A.M. Popescu, E. Radu, Effects of Organic Carbon Sources on Cell Growth and Oil Content of Microalgae Species, International Conference RICCCE 19, Sibiu, Romania, 2-5 septembrie 2015.
  • Sanda VELEA “Evaluation of electroflocculation for microalgae harvesting”, 22nd European Biomass Conference and Exhibition CCH – Congress Center Hamburg, Germany -23-26 June 2014

Papers:

  • Andreea Dragu, Solen Kinayyigit, Eduardo J. García-Suárez, Mihaela Florea, Emil Stepan, Sanda Velea, Liviu Tanase, Vincent Collière, Karine Philippot, P. Granger, Vasile I. Pârvulescu, “Deoxygenation of oleic acid: Influence of the synthesis route of Pd/mesoporous carbon nanocatalysts onto their activity and selectivity”, Applied Catalysis A: General, vol. 504, pag. 81-91 anul 2015, (ISSN: 0926860X)
  • Velea S., Ilie L., Stepan E., Chiurtu R., „New Photobioreactor Design for Enhancing the Photosynthetic Productivity of Chlorella homosphaera Culture”, “Revista de Chimie”, 2014, 65(1), 56-60, 2014, (ISSN 0034-7752)
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Dr. Dorinde Kleinegris

Dr. Dorinde Kleinegris WG Member

Dr. Dorinde Kleinegris

WG Member

Dr. Dorinde Kleinegris

Country: Norway

Institution: Uni Research AS

Email: dorinde.kleinegris@uni.no

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Dr John C Dodd

Dr John C Dodd WG Member

Dr John C Dodd

WG Member

Dr John C Dodd

Country: UK

Institution: AlgaeCytes Ltd

Email : johndodd@algaecytes.com

Website: www.algaecytes.com



Key investigations:

  • Omega 3 (EPA) algal oils commercialisation

  • New microalgal products for the nutraceutical and cosmeceutical markets

  • Bioprocessing/biorefining

Key publications:

 

  1. AlgaeCytes has patent application (WO2012101459 A3) on continuous phototrophic algal production of a specific Omega oil (EPA) co-authored by Dr. Dodd.

  1. DODD, J.C., BURTON, C., BURNS, R.G. & JEFFRIES, P. (1987). Soil phosphatase activity around roots of three host plants infected with VAM fungi. New Phytologist 107, 163‑172.

  2. DODD, J.C., GIANINAZZI-PEARSON, V., ROSENDAHL, S. & WALKER, C. (1994) European Bank of Glomales – An essential tool for efficient international and interdisciplinary collaboration. In: Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems. Eds. S. Gianinazzi and H. Schuepp. Birkhauser Press, Basel, Switzerland. pp 41-46.

  3. DODD, J.C. & ROSENDAHL, S. (1996) The BEG Expert System – A multi media identification system for arbuscular mycorrhizal fungi. Mycorrhiza 6, 275-278.

  4. DODD, J.C., PRUSKY, D. & JEFFRIES, P. (1997) Fruit Diseases. In: The Mango. (Ed.) R.E.Litz. CAB International Press, Wallingford, Oxon., UK. pp. 257-280.

  5. DODD, J.C., BODDINGTON, C.L, RODRIGUEZ, A., GONZALEZ-CHAVEZ, C. & MANSUR, I. (2000) Mycelium of Arbuscular Mycorrhizal fungi (AMF) from different genera: form, function and detection. Plant and Soil 226:131-151.

  6. JEFFRIES, P. & DODD, J.C. (2000) Molecular Ecology of Mycorrhizal Fungi. In: Applied Microbial Systematics Eds. Priest, F.G., Goodfellow, M. Kluwer Academic Publishers, Dordrecht. pp. 73-105.

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Maria Jose Fabra

Maria Jose Fabra WG Member

Maria Jose Fabra

WG Member

Maria Jose Fabra

Country: Spain

Institution: IATA-CSIC

Email: mjfabra@iata.csic.es

Website: http://www.iata.csic.es/

Key investigations:

  • Development of ecofriendly bionanocomposites films
  • Development of active biodegradable food packaging materials
  • Encapsulation of active and bioactive compounds

Key publications:

  • J. Fabra, A. López-Rubio, J.M. Lagaron (2016). Use of the electrohydrodynamic process to develop active/bioactive bilayer films for food packaging applications. Food Hydrocolloids, 55, 11-18.
  • L. Castro-Mayorga, M.J. Fabra, J.M. Lagaron (2016). Stabilized nanosilver based antimicrobial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanocomposites of interest in active food packaging. Innovative Food Science and Emerging Technologies, doi:10.1016/j.ifset.2015.10.019
  • J. Fabra, A. López-Rubio, J.M. Lagaron (2015). Three-Layer Films Based on Wheat Gluten and Electrospun PHA. Food and Bioprocess Technology, 8, 2330-2340.
  • J. Fabra, A. López-Rubio, J.M. Lagaron (2015). Three-Layer Films Based on Wheat Gluten and Electrospun PHA. Food and Bioprocess Technology, 8, 2330-2340.
  • Ambrosio-Martín, M.J. Fabra, A. López-Rubio, J.M. Lagaron (2015). Melt polycondensation to improve the dispersion of bacterial cellulose into polylactide via melt compounding: Enhanced barrier and mechanical properties. Cellulose, 22, 1201-1226.
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Dr Gloria Sánchez

Dr Gloria Sánchez WG Member

Dr Gloria Sánchez

WG Member

Dr Gloria Sánchez

Country: Spain

Institution: University of Valencia/ IATA-CSIC

Email: gloriasanchez@iata.csic.es   

Website: https://www.iata.csic.es/es/personal/gloria-sanchez-moragas

Key investigations:

  • Evaluation of antimicrobial (antiviral and antibacterial) activity of natural compounds
  • Development of virus detection methods in food

Key publications:

  • J. Fabra, J.L. Castro-Mayorga, W. Randazzo, J.M. Lagarón, A. Lopez-Rubio, R. Aznar, G. Sánchez. Efficacy of cinnamaldehyde against enteric viruses and its activity after incorporation into biodegradable multilayer systems of interest in food packaging. Food and Environmental Virology DOI: 10.1007/s12560-016-9235-7
  • G. Sánchez, R. Aznar. Evaluation of natural compounds of plant origin for inactivation of enteric viruses. Food and Environmental Virology 7:183-187 (2015).
  • Sánchez, R. Aznar, G. Sánchez. The effect of carvacrol on enteric viruses. International Journal Food Microbiology 192:72-76 (2015).
  • Azizkhanib, P. Elizaquível, G. Sánchez, M.V. Selma & R. Aznar. Comparative efficacy of Zataria multiflora Boiss., Origanum compactum and Eugenia caryophyllus essential oils against E. coli O157:H7, feline calicivirus and endogenous microbiota in commercial baby-leaf salads. International Journal Food Microbiology 166:249-255 (2013).
  • Elizaquível, M. Azizkhanib, G. Sánchez & R. Aznar. Evaluation of Zataria multiflora Boiss. essential oil activity against Escherichia coli O157:H7, Salmonella enterica and Listeria monocytogenes by propidium monoazide quantitative PCR in vegetables. Food Control 34:774-776 (2013)
  • Martínez-Abad, G. Sánchez, J. M. Lagarón, M. J. Ocio. Characterization and antibacterial activity of polycaprolactone films containing cinnamaldehyde. Food Control 3:214.220 (2013)
  • Fernández-Saiz, G. Sánchez, C. Soler, J. M. Lagarón, M. J. Ocio. Chitosan Films for the Microbiological Preservation of Refrigerated Sole and Hake fillets. Food Control 34:61-68 (2013)
  • Martínez-Abad, J. M. Lagarón, M. J. Ocio, G. Sánchez. Evaluation of silver-infused polylactide films for inactivation of Salmonella and feline calicivirus in vitro and on fresh-cut vegetables. International Journal of Food Microbiology 162: 89- 94 (2013)
  • Elizaquível, M. Azizkhanib, R. Aznar & G. Sánchez. The Effect of Essential Oils on norovirus surrogates Food Control 32: 275-278 (2013)
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Dr Tina Silovic

Dr Tina Silovic WG Member

Dr Tina Silovic

WG Member

Dr Tina Silovic

Country: Croatia

Institution: Rudjer Boskovic Institute

Email: tina.silovic@cim.irb.hr

Websites:       

 http://tina-silovic.hr

http://www.irb.hr/eng/People/Tina-Silovic

Key investigations:

  • I am collaborator in the Croatian national project: “ Assessment of Adriatic Algae Potential in co-generation production of 3rd generation biofuel”
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Dr Zivan Gojkovic

Dr Zivan Gojkovic WG Member

Dr Zivan Gojkovic

WG Member

Dr Zivan Gojkovic

Country: Sweden

Institution: Umea University, Sweden

Email: zivan.gojkovic@umu.se; gojkoviczivan@gmail.com

Key investigations:

  • Umea University, Sweden. Post-doc on a Biorafinery project (March 2016-present)
  • AlgaePARC – Algae Production and Research Center of The Wageningen University, The Netherlands. Work on comparison of lipid production in various microalgae species grown in vertical photobioreactors outdoors (May-December 2015)
  • Algal Biotechnology Group. University of Huelva, Spain. Junior researcher. Grant from Agrifood Campus of International Excellence (ceiA3) for foreign PhD-students. (October 2013-March 2014)

Key publications:

  • Gojkovic Z, Vilchez C, Marova I, Gomez-Ariza JL, Garbayo-Nores I, Selenium bioaccumulation and toxicity in cultures of green microalgae. Algal Research, 2015, Vol. 7,pp. 106-116.
  • Gojkovic Z, Vilchez C, Torronteras R, Vigara J, Gomez-Jacinto V, Janzer N, Gomez-Ariza JL, Marova I, Garbayo-Nores I, Effect of selenate on viability and selenomethionine accumulation of Chlorella sorokiniana grown in batch culture. The Scientific World Journal, 2014, http://dx.doi.org/10.1155/2014/401265
  • Gojkovic Z, Garbayo-Nores I, Gomez-Jacinto V, Garcia-Barrera T, Gomez-Ariza JL, Marova I, Vilchez-Lobato C, Continuous production of selenomethionine-enriched Chlorella sorokiniana biomass in a photobioreactor. Process Biochemistry, 2013, vol 48, pp. 1235-1241.
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Dr. Giuseppe Torzillo

Dr. Giuseppe Torzillo WG Member

Dr. Giuseppe Torzillo

WG Member

Dr. Giuseppe Torzillo

Country: Italy

Institution: Istituto per lo Studio degli Ecosistemi del CNR

Email: torzillo@ise.cnr.it

Website: http://www3.ise.cnr.it

Key investigations:

  • Microalgal biotechnology
  • Microalgal physiology
  • Photobiological hydrogen production
  • Bioenergy
  • Photobioreactors

 Key publications:

  • Silva M., Torzillo G. (2012). Nitrogen and phosphorus removal through laboratory batch cultures of Chlorella vulgaris and cyanobacterium Plankthotrix isothrix grown as monoalgal and as co-cultures. J. Appl. Phycol. 24: 267-276.
  • Scoma A., Giannelli L Faraloni C., Torzillo G. (2012). Outdoor H2 production in a 50-liter tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii . J. Biotechnol. 157::620-627
  • Scoma A., Giannelli L Faraloni C., Torzillo G. (2012) Sustained H2 production in a Chlamydomonas. reinhardii D1 protein mutant. J. Biotechnol. 157: 613-619;
  • Scarsella M., Torzillo G., Cicci A., Belotti G., De Filippis P., Bravi M. (2012). Mechanical stress tolerance of two microalgae. Process Biochemistry 47/11:1606-1614.
  • Samori C., Torri C., Fabbri D., Falini G., Faraloni C., Galletti P., Spera S., Taglisavini E., Torzillo G. (2012). Unusual catalysts from molasses: synthesis, properties and application in obtaining of biofuels from algae. ChemSusChem 5: 1501-1512.
  • Adessi A., Torzillo G. Baccetti E., De Philippis R. (2012). Sustained outdoor H2 production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor. Int. J. Hydrogen Energy, 37: 8840-8849.
  • Torzillo G., Faraloni C., Silva A.M., Kopecky J., Pilny J., Masojidek J. (2012). Photoacclimation of Phaeodactlylum tricornutum (Bacillariophyceae) cultures grown outdoors in photobioreactors and open ponds. Eur J Phycol. 47: 169-181.
  • Giannelli L., Torzillo G. (2012). Hydrogen production with the microalga Chlamydomonas reinhardtii grown in a compact tubular photobioreactor immersed in a scattering nanoparticle suspension. Int. J. Hydrogen Energy 37: 16951-16961.
  • Silva Benavides A. M., Torzillo G., Kopecky J., Masojidek J. (2013) Productivity and biochemical composition of Phaeodactlylum tricornutum (Bacillariophyceae) cultures grown outdoors in tubular photobioreactors and open ponds. Biomass and Bioenergy 54:115-122.
  • Arias-Peñaranda M. T., Cristiani-Urbina E., Montes-Horcasitas, C.,  Esparza-Garcı́a F, Torzillo G., Cañizares-Villanueva, R.O. (2013). Scenedesmus incrassatulus CLHE-Si01: a potential source of renewable lipid for high quality biodiesel production. Bioresource Technolology. 140: 158-164.
  • Faraloni C., Torzillo G. (2013). Xanthophyll cycle induction by anaerobic conditions under low light in Chlamydomonas reinhardtii. Appl. Phycol. 25: 1457-1471.
  • Martínez-Roldán A.J., Perales-Vela H.V., Cañizares-Villanueva R.O., Torzillo G. (2014). Physiological response of Nannochloropsis sp., to saline stress in laboratory bath cultures. J. Appl. Phycol. 26:115-121
  • Torzillo G., Scoma A., Faraloni C., Giannelli L. (2014) Advances in the biotechnology of hydrogen production with the microalga Chlamydomonas reinhardtii. Crit. Review in Biotechnol. DOI: 10.3109/07388551.2014.900734.
  • Oncel S.S., Kose A., Faraloni C., Imamoglu E., Elibol M., Torzillo G., Vardan Sukan F. (2014). Biohydrogen production using mutant strains of Chlamydomonas reinhardtii: The effects of light intensity and illumination patterns. Biochem Eng. J. 92: 47-52.
  • Oncel S.S., Kose A., Faraloni C., Imamoglu E., Elibol M., Torzillo G., Vardan Sukan F. (2015). Biohydrogen production with the model alga Chlamydomonas reinhardtii: A simulation of environmental conditions for outdoor experiments. Int. J. Hydrogen Energy (http://dx.doi.org/10.1016/j.ijhydene.2014.12.121.
  • Stojkovic D., Torzillo G., Faraloni C., Valant M. (2015) Hydrogen production by sulfur-deprived TO2-encapsulated Chlamydomonas reinhardtii cells. Int. J. Hydrogen Energy, 40:3201-3206.

 

Chapter in books:

  • Torzillo G. Vonshak A., (2013). Environmental stress physiology with reference to mass cultures. In: Handbook of Microalgal Cultures (Richmond A., Hu Q. Eds.). Blackwell Science Ltd. Oxford.
  • Torzillo G., Seibert M. Hydrogen production by Chlamydomonas reinhardtii (2013) Handbook of Microalgal Cultures (Richmond A., Hu Q. Eds.). Blackwell Science Ltd. Oxford.

 

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Dr Patrick Murray

Dr Patrick Murray WG Member

Dr Patrick Murray

WG Member

Dr Patrick Murray

Country: Ireland

Institution: Shannon Applied Biotechnology Centre, Limerick Institute of Technology

Email: patrick.murray@lit.ie

Website: www.lit.ie; www.shannonabc.ie

 
Key investigations:

  • Scientific Coordinator Leader Project title: BAMMBO-Biologically active molecules of marine based origin. Funding body: European Union FP7 Cooperation-Biotechnology-KBBE Grant
  • Principal Investigator Project title: Environmentally sustainable organic processes for the enclosed production of omega-3 polyunsaturated fatty acids (PUFA’s) and carotenoids (e.g. astaxanthin) from microalgae

Key publications:

  • Effect of macro- and micro-nutrient limitation on superoxide dismutase activities and carotenoid levels in microalga Dunaliella salina CCAP 19/18 2013 Bioresource Technology Vol. 147 p 23-28 Sushanta Kumar Saha, Siobhan Moane, Patrick Murray.
  • Life cycle assessment of the production of the red antioxidant carotenoid astaxanthin by microalgae: from lab to pilot scale 2013 Journal of Cleaner Production 1Paula Pérez-López Sara González-García, Clayton Jeffryes, Spiros N. Agathos, Edward McHugh, Daniel Walsh, Patrick Murray, Siobhan Moane, Gumersindo Feijoo, Ma Teresa Moreira
  • Sustainable production of biologically active molecules of marine based origin 2013 New Biotechnology Volume 30, Issue 6, 25 September 2013, Pages 839 Patrick M. Murray1,2 et al.
  • Tagging of biomolecules with deuterated water (D2O) in commercially important microalgae 2013 Biotechnology Letters Jul;35(7):1067-72.Sushanta Kumar Saha, Jeremiah Hayes, Siobhan Moane, Patrick Murray
  • Effect of various stress-regulatory factors on biomass and lipid production in microalga Haematococcus pluvialis (2012) Bioresource Technology 128: 118-124.Sushanta Kumar Saha, Edward McHugh, Jeremiah Hayes, Siobhan Moane, Daniel Walsh, Patrick Murray
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Dr Cristina González-Fernández

Dr Cristina González-Fernández WG Member

Dr Cristina González-Fernández

WG Member

Dr Cristina González-Fernández

Country: Spain

Institution: IMDEA Energy

Email: cristina.gonzalez@imdea.org

Website: http://www.energy.imdea.org/

Key investigations:

  • Anaerobic Digestion

  • Wastewater bioremediation

  • Photosynthetic microorganisms

Key publications:

  • C. Gonzalez-Fernandez, B. Sialve, B. Molinuevo-Salces “Anaerobic digestion of microalgal biomass: Challenges, opportunities and research needs” Bioresource Technology 198 (2015) 896-906

  • L. Mendez, A. Mahdy, M. Ballesteros, C. Gonzalez FernandezChlorella vulgaris vs cyanobacterial biomasses: Comparison in terms of biomass productivity and biogas yield” Energy Conversion and Management 92 (2015) 137 – 142

  • Mahdy, L. Mendez, S. Blanco, M. Ballesteros, C. Gonzalez “Protease cell wall degradation of Chlorella vulgaris: Effect on methane production” Bioresource Technology 171 (2014) 421 – 427

  • C. Gonzalez Fernandez, B. Sialve, N. Bernet, J.P. Steyer “Impact of microalgae characteristics on their conversion to biofuel. Part II: Focus on biomethane production” Biofuels Bioproducts & Biorefining 6 – 2 (2012) 205 – 218

  • C. Gonzalez Fernandez, B. Molinuevo Salces, M.C. Garcia Gonzalez “Nitrogen transformationsunder different conditions in open ponds by means of microalgae-bacteria consortium treating pig slurry” Bioresource Technology 102 (2011) 960 – 966

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Amparo Lopez Rubio

Amparo Lopez Rubio WG Member

Amparo Lopez Rubio

WG Member

Amparo Lopez Rubio

Country: Spain

Institution: Institute of Agrochemistry and Food Technology (IATA-CSIC)

Email: amparo.lopez@iata.csic.es

Website: www.iata.csic.es 

Key investigations:

  • Development of materials for food applications
  • Bioactive micro- and nanoencapsulation
  • Structure-function correlation studies

Key publications:

  • G. Gómez-Mascaraque, A. López-Rubio* (2016). Protein-based emulsion electrosprayed micro- and submicroparticles for the encapsulation and stabilization of thermosensitive hydrophobic bioactives. Journal of Colloid and Interface Science 465, 259-270.
  • G. Gómez-Mascaraque, J.M. Lagaron, A. Lopez-Rubio* (2015). Electrosprayed gelatin submicroparticles as edible carriers for the encapsulation of polyphenols of interest in functional foods. Food Hydrocolloids 49, pp.42-52.
  • Martínez-Sanz, A. López-Rubio, J.M. Lagaron (2012). Optimization of the dispersion of unmodified bacterial cellulose nanowhiskers into polylactide via melt compounding to significantly enhance barrier and mechanical properties. Biomacromolecules, 13, 3887-3899.
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Dr Müge ISLETEN HOSOGLU

Dr Müge ISLETEN HOSOGLU WG Member

Dr Müge ISLETEN HOSOGLU

WG Member

Dr Müge ISLETEN HOSOGLU

Country: Turkey

Institution: Canakkale Onsekiz Mart University Food Eng. Dept.

Email: mugehosoglu@comu.edu.tr

Website: http://aves.comu.edu.tr/mugehosoglu/

Key investigations:

  • Algal biotechnology
  • Food
  • Bioprocess Engineering

Key publications:

  • Yıldırım, A., Demirel, Z., Isleten-Hosoglu, , Akgun, İ. H., Hatipoglu-Uslu, S., Conk-Dalay, M. 2014. Carotenoid and Fatty Acid Compositions of an Indigenous Ettlia texensis Isolate (Chlorophyceae) Under Phototrophic and Mixotrophic Conditions. Applied Biochemistry and Biotechnology. 172(3):1307-19.
  • Isleten-Hosoglu, , Ayyıldız-Tamis, D., Zengin, G., Elibol, M. 2013. Enhanced Growth and Lipid Accumulation by a New Ettlia texensis Isolate Under Optimized Photoheterotrophic Condition. Bioresource Technology. 131, 258-265
  • Isleten-Hosoglu, , Gultepe, I., Elibol, M. 2012. Optimization of Carbon and Nitrogen Sources for Biomass and Lipid Production by Chlorella saccharophila under Heterotrophic Conditions and Development of Nile Red Fluorescence Based Method for Quantification of Its Neutral Lipid Content. Biochemical Engineering Journal, 61, 11–19.

 

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Dr Judita Koreivienė

Dr Judita Koreivienė WG Member

Dr Judita Koreivienė

WG Member

Dr Judita Koreivienė

Country: Lithuania

Institution: Laboratory of Algology and Microbial Ecology, Nature Research Centre

Email: judita.koreiviene@gmail.com 

Key investigations:

  • Diversity and ecology of freshwater/brackish algae and cyanobacteria
  • Trophic interactions in aquatic ecosystems
  • Microalgae and cyanobacteria isolation and culture maintenance
  • Microalgae-based wastewater treatment
  • Cell wall disruption

 Key publications:

  • Koreivienė J., Kasperovičienė J., Savadova K., Karosienė J., Vitonytė I., 2016. Collection of pure cultures of algae and cyanobacteria for research, teaching and biotechnological applications (Nature Research Centre, Lithuania). Botanica Lithuanica, 22(1): 87–92.
  • Šulčius S., Alzbutas G., Kvederavičiūtė K., Koreivienė J., Zakrys L., Lubys A., Paškauskas R. 2015. Draft genome sequence of the cyanobacterium Aphanizomenon flos-aquae strain 2012/KM1/D3, isolated from the Curonian Lagoon (Baltic Sea). Genome Announc. 3(1):e01392-14. doi:10.1128/genomeA.01392-14.
  • Anne O., Bugajev D., Koreiviene J., 2015. Determining optimal growth conditions for the highest biomass microalgae species in Lithuanian part of the Curonian Lagoon for further cultivation. International Journal of Environmental Research, 9(1): 233–246.
  • Koreivienė J., Anne O., Kasperovičienė J., Burškytė V. Cyanotoxin management and human health risk mitigation in recreational waters. Environmental Monitoring and Assessment, 186:4443–4459.
  • Koreivienė J., Valčiukas R., Karosienė J., Baltrėnas P., 2014. Testing of Chlorella/Scenedesmus microalgae consortia for remediation of wastewater, CO2 mitigation and algae biomass feasibility for lipid production. Journal of Environmental Engineering and Landscape Management, 22(02): 105–114.
  • Seppälä J., Spilling K., Manninen K., Salo E., Cahill B., Gröndahl F., Pechsiri J. S., Christensen P. B., Belous O., Koreiviene J., Olenina I. 2012. Large-scale microalgae cultivation (pp. 125-145). In: Schultz-Zehden A. and Matczak M. (Eds.), An assessment of innovative and sustainable uses of Baltic marine resources. Maritime Institute in Gdańsk. Available on the website: http://www.submariner-project.eu/index.php?option=com_content&view=article&id=233&Itemid=384

 

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Dr Elena Ficara

Dr Elena Ficara WG Member

Dr Elena Ficara

WG Member

Dr Elena Ficara

Country: Italy

Institution: Politecnico di Milano, Department of Civil and Environmental Engineering (DICA)

Email: elena.ficara@polimi.it  

Websites:

www.fabbricabioenergia.polimi.it/microgate  

http://www.imap-project.it/

Key investigations:

  • MicroGate – Use of MICROalgae to mitiGATE nitrogen pollution from agricultural wastewaters (03/2015 – 07-2017). – Founding: Fondazione Cariplo (fabbricabioenergia.polimi.it/microgate)
  • IMAP – Integration of microalgal based processes in wastewater treatment (IMAP) – Founding: Fondazione Cariplo (06/2016-12/2018) (http://www.imap-project.it/)
  • Il polo delle microalghe (the microalgal knowledge park). – Founding: Fondazione Cariplo (09/2016 – 09/2019)
  • VADEMECUM (VAlorizzazione e DEpurazione di reflui e scarti agroindustriali MEdiante la Creazione di Una bioraffineria a base Microalgale) – Founding: Italian Ministery of University and Research.

Key publications:

  • Marazzi F., Sambusiti C., Monlau F., Cecere S. E., Scaglione D., Barakat A., Mezzanotte V., Ficara E. (2017). A novel option for reducing the optical density of liquid digestate to achieve a more productive microalgal culturing. Algal Research (in press).
  • Marazzi, F., Ficara, E., Fornaroli, R., & Mezzanotte, V. (2017). Factors Affecting the Growth of Microalgae on Blackwater from Biosolid Dewatering. Water, Air, & Soil Pollution, 228(2), 68.
  • Monlau, F., Sambusiti, C., Ficara, E., Aboulkas, A., Barakat, A., Carrère, H. (2015), New opportunities for agricultural digestate valorization: Current situation and perspectives. Energy and Environmental Science, 8 (9), pp. 2600-2621. 
  • Ficara, E., Uslenghi, A., Basilico, D., Mezzanotte, V. (2014). Growth of microalgal biomass on supernatant from biosolid dewatering. Water Science and Technology, 69 (4), pp. 896-902.
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Dr. Luísa Gouveia

Dr. Luísa Gouveia WG Member

Dr. Luísa Gouveia

WG Member

Dr. Luísa Gouveia

Country: Portugal

Institution: LNEG – National Laboratory of Energy and Geology, IP.

Email: luisa.gouveia@lneg.pt

Website: www.lneg.pt

Key investigations:

  • Microalgae_food_ feed
  • Lipid and pigment induction
  • Biofuel-based microalgae (H2, biogas, bioethanol, biodiesel)
  • Wastewater treatment
  • CO2 mitigation
  • Supercritical CO2 extraction of bioactive high-value compounds from microalgae
  • Microalgae biomass harvesting
  • Microalgae Biorefineries
  • Life Cycle Analysis

 Key publications:

  • Miranda, J.R.; Passarinho P.C.; Gouveia L. (2012). Pre-treatment optimization of Scenedesmus obliquus biomass for bioethanol production. Bioresource Technology 104, 342-348.
  • Matos, C.; Santos, M.; Nobre, B.; Gouveia, L. (2013). Nannochloropsis biomass recovery by Electro-Coagulation for Biodiesel and Pigment production. Bioresource Technology 134, 219-226.

 

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Dr AYSENUR UGURLU

Dr AYSENUR UGURLU WG Member

Dr AYSENUR UGURLU

WG Member

Dr AYSENUR UGURLU

Country : Turkey

Institution : Hacettepe University

Email: ugurlu@hacettepe.edu.tr

Website: http://www.cevre.hacettepe.edu.tr/english/prs/sayfalar/Aysenur/Aysenur.shtml

Key investigations:

  • Biogas production from wastes
  • Environmental biotechnology
  • Nutrient removal from wastewater

Key publications:

  • A. Varol; A. Ugurlu, “Biogas Production from Microalgae (Spirulina platensis) in a Two Stage Anaerobic System”, WAVE
  • Dalkilic, K; Ugurlu, A, “Biogas production from chicken manure at different organic loading rates in a mesophilic-thermopilic two stage anaerobic system”, J BIOSCI BI, V 120, N 3, 315-322, 2015
  • E.Agayev;A.Ugurlu, “Biogas production from Horse Dung”,
  • Kayranli, B; Ugurlu, A, “ASSESSMENT OF METHANOGENIC ACTIVITY AND KINETICS OF ANAEROBIC GRANULAR SLUDGE”, FR ENV BULL, V21, N 8, 2394-2398, 2012
  • Kayranli, B; Ugurlu, A, “Effects of temperature and biomass concentration on the performance of anaerobic sequencing batch reactor treating low strength wastewater”, DESALINATION, V 278, N 1, 77-83, 2011
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Dr Poul Erik Jensen

Dr Poul Erik Jensen WG Member

Dr Poul Erik Jensen

WG Member

Dr Poul Erik Jensen

Country: Denmark

Institution: Copenhagen Plant Science Centre, Dept. Plant and Environmental Sciences, University of Copenhagen.

Email: peje@plen.ku.dk

Website: http://plen.ku.dk/english/research/molecular_plant_biology/photosyn/

Key investigations:

  • Synthetic biology using photosystem I as a light-driven reductase.

  • Elucidation of biosynthetic pathways leading to chlorophyll and plant sterols.

  • The use of mutants to unravel basic mechanisms in photosystem function

  • Molecular understanding of function and regulation of photosynthetic complexes

 

Key publications:

  • Włodarczyk A, Gnanasekarana T, Nielsen AZ, Zulu NN, Mellor SB, Thøfner JFB, Olsen CE, Mottawie MS, Burow M, Luckner M, Pribil M, Feussner I, Møller BL, and Jensen PE (2016). Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803. Metabolic Engineering 33: 1-11. doi: 10.1016/j.ymben.2015.10.009.

  • Gangl D, Zedler JAZ, Rajakumar PD, Martinez EMR, Riseley A, Włodarczyk A, Purton S, Sakuragi Y, Howe CJ, Jensen PE, Robinson C (2015). Biotechnological exploitation of microalgae. J. Exp. Bot. doi: 10.1093/jxb/erv426.

  • Lassen, LM, Zygadlo Nielsen A, Friis Ziersen BE; Gnanasekaran T, Møller BL, Jensen PE (2014). Redirecting photosynthetic electron flow into light-driven synthesis of alternative products including high-value bioactive natural compounds. ACS Synth. Biol. 3(1): 1–12. DOI: 10.1111/ppl.12108

  • Lassen LMM, Nielsen AZ, Olsen CE, Bialek W, Jensen K, Møller BL, Jensen PE (2014). Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis. PLoS ONE 9(7): e102184. doi:10.1371/journal.pone.0102184

  • Zygadlo Nielsen A, Friis Ziersen BE, Jensen K, Lassen LM, Olsen CE, Møller BL, Jensen PE (2013). Redirecting photosynthetic reducing power towards bioactive natural product synthesis. ACS Synthetic Biology 2: 308−315. dx.doi.org/10.1021/sb300128r.



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 Wolfgang Frey

Wolfgang Frey WG Member

Wolfgang Frey

WG Member

 Wolfgang Frey

Country: Germany

Institution: KIT / Institute for Pulsed Power and Microwave Technology (IHM)

Email: wolfgang.frey@kit.edu

Website: https://www.ihm.kit.edu/english/299.php

Key investigations:

  • Component extraction from microalgae by pulsed electric field treatment
  • Basic mechanisms of membrane permeabilization by pulsed electric fields (PEF)

Key publications:

  • Goettel, C. Eing, C. Gusbeth, R. Straessner, and W. Frey, “Pulsed electric field assisted extraction of intracellular valuables from microalgae,” Algal Research-Biomass Biofuels and Bioproducts, vol. 2, pp. 401-408, Oct 2013
  • Eing, M. Goettel, R. Straessner, C. Gusbeth, and W. Frey, “Pulsed Electric Field Treatment of Microalgae-Benefits for Microalgae Biomass Processing,” IEEE Transactions on Plasma Science, vol. 41, pp. 2901-2907, Oct 2013.
  • Kotnik, W. Frey, M. Sack, S. Haberl Meglič, M. Peterka, and D. Miklavčič, “Electroporation-based applications in biotechnology,” Trends in Biotechnology, vol. 33, pp. 480-488, 2015.



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Prof Javier Raso Pueyo

Prof Javier Raso Pueyo WG Member

Prof Javier Raso Pueyo

WG Member

Prof Javier Raso Pueyo

Country: Spain

Institution: Food Technology Unit, Faculty of Veterinary, University of Zaragoza

Email: jraso@unizar.es

Website: http://www.unizar.es/cta/raso.htm

Key investigations:

  • Process optimization to electroporate microalgae by pulsed electric fields
  • Mechanims of microalgae electroporation by pulsed electric fields
  • Improving pigment extraction from microalgae by pulsed electric field

Key publications:

  • Luengo E, Condon-Abanto S, Alvarez I, Raso J Effect of Pulsed Electric Field Treatments on Permeabilization and Extraction of Pigments from Chlorella vulgaris (2014). The Journal of Membrane Biology 247:1269-77
  • Luengo E, Martínez JM, Coustets M, Álvarez I, Teissié, J, Rols MP and  Raso J (2015). A comparative study on the effects of millisecond- and microsecond-pulsed electric field treatments on the permeabilization and extraction of pigments from Chlorella vulgaris The Journal of Membrane Biology 248:883-91.
  • Luengo, E., Martínez, J.M., Bordetas, A., Álvarez, I. & Raso, J. (2015) Influence of the treatment medium temperature on lutein extraction assisted by pulsed electric fields from Chlorella vulgaris. Innovative food science and emerging technologies. 29, 15-22.
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Dr Marta Martinez Sanz

Dr Marta Martinez Sanz WG Member

Dr Marta Martinez Sanz

WG Member

Dr Marta Martinez Sanz

Name: Marta Martinez Sanz 

Country: Spain

Institution: Institute of Agrochemistry and Food Technology (IATA-CSIC)

Email: mmartinez@iata.csic.es

Website: https://www.iata.csic.es

Key investigations:

  • Extraction and characterisation of cellulose from different resources
  • Development of biobased nanocomposite materials
  • Investigation of cellulose interactions with plant cell wall components
  • Application of scattering techniques to study the structure of biopolymers

 Key publications:

  • Martínez-Sanz, D. Mikkelsen, B. Flanagan, M.J. Gidley, E.P. Gilbert (2016) Multi-scale model for the hierarchical architecture of native cellulose hydrogels. Carbohydrate Polymers 147, 542-555.
  • Martínez-Sanz, P. Lopez-Sanchez, M.J. Gidley, E.P. Gilbert (2015) Evidence for differential interaction mechanism of plant cell wall matrix polysaccharides in hierarchically-structured bacterial cellulose. Cellulose, 22, 1541-1563.
  • Martínez-Sanz, A. López-Rubio, J.M. Lagaron (2012). Optimization of the dispersion of unmodified bacterial cellulose nanowhiskers into polylactide via melt compounding to significantly enhance barrier and mechanical properties. Biomacromolecules, 13, 3887-3899.
  • Martínez-Sanz, A. Lopez-Rubio, J.M. Lagaron (2011) Optimization of the nanofabrication by acid hydrolysis of bacterial cellulose nanowhiskers. Carbohydrate Polymers, 85, 228-236.
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Dr Raja Rathinam

Dr Raja Rathinam WG Member

Dr Raja Rathinam

WG Member

Dr Raja Rathinam

Country: Portugal

Institution: University of Algarve

Email: rraja307@yahoo.co.in

Website: http://w3.ualg.pt/~icarva/group.html

Key investigations:

  • Post-Doctoral Research (Biofuel from microalgae) at Department of Biotechnology, Indian Institute of Technology Madras (IIT M), Chennai, INDIA

Phytoplankton screening from various fresh and marine water sources for biofuel. Few microalgae (Amphiprora sp., Chaetoceros wighami, Chlorella sp., Dunaliella parva, Scenesdesmus sp. and Spirulina platensis) were isolated and maintained under laboratory conditions in different medium such as F/2, De Walne’s and Zarrouk’s. Effect of different parameters like salt concentration of growth medium, inoculum efficiency, effect of seawater on biomass production, effect of oxidation pond water available in IIT M, biomass difference between algae rack and shake flask cultures, culture grown in 12/12, 18/6, 6/18, 24 hours light/dark and light intensity on total lipid content was monitored. FAME analysis and fatty acid profiles were determined through GC-MS. The biomass of Chlorella sp. and Chaetoceros sp. are scaled-up in a 15 li photobioreactor under different stress conditions as mentioned above. The lipid extractions were done by Bligh and Dyer method.

  • Lead Scientist, Pilot scale operations (indoor), Aquatic Energy, LLC, Lake Charles, Louisiana, USA

Apart from laboratory experience, I have maintained algal cultures in seven different sized open raceway ponds. The pH, temperature, contamination level, cell count and OD values were taken three times a day. Depending upon the situation, nutrients were added near the paddle wheels. During summer, every day the pH were shoot-up in the morning to bring it to normal, CO2 were supplied to the ponds. According to water evaporation level every day fresh water was added to meet the deficit especially on summer. On winter, the increased water level was calculated and nutrients were added to keep the culture healthy. Every day, the biomass were harvested in a larger quantity.

Key publications:

  • Rathinam Raja, Hemaiswarya S, V. Ganesan and Isabel S Carvalho, 2016. Recent developments in therapeutic applications of cyanobacteria. Critical Reviews in Microbiology 42(3): 394-405.
  • Raja R, Hemaiswarya S, Isabel S. Carvalho and K. Arunkumar, Impact of salt effluent and seaweed liquid fertilizers on three microalgae. Brazilian Journal of Botany 38(3): 547-553.
  • Hemaiswarya S, Raja, Isabel S.Carvalho, R. Ravikumar, Vasudeo Zambare and Debmalya Barh, 2012. An Indian scenario on renewable and sustainable energy sources with emphasis on algae. Applied Microbiology and Biotechnology, 96: 1125-1135.
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